A Simple Method for Serially Sectioning Small Specimens of Hard Tissue

A simple method for orientating small objects for sectioning, with special regard to nematodes.

FOR solving certain anatomical problems in free-living nematodes serial sections are necessary. Various methods of orientation proved unsatisfactory for these small objects (in this case 700^ in length and 30/x in width). The following method was worked out and found excellent: In a drop of water the nematode is heat-paralysed, stretched at 50-6o° C , and pipetted into Bouin's mixture (Graupner...

A RAPID AND SIMPLE METHOD OF DNA EXTRACTION FROM CLINICAL SPECIMENS CONTAINING MYCOBACTERIUM LEPRAE FOR PCR ASSAYS

We describe a rapid, simple, and reliable procedure for routine isolation of Mycobacterium leprae DNA from slit-skin swab specimens. This one-step DNA extraction method is based on the utilization of ChelexR 100, a chelating ion exchange resin. Slit-skin swab specimens from seven untreated leprosy patients at Baba Baghi Leprosy Hospital in Iran were processed by this procedure. The polymer...

A convenient Simple Method for Synthesis of Meta-iodobenzylguanidine (MIBG)

Radioiodinated meta iodobenzylguanidine (MIBG) is one of the important radiopharmaceuticals in Nuclear Medicine. [123/131I]MIBG is used for imaging of Adrenal medulla, studying heart sympathetic nerves, treatment of pheochromacytoma and neuroblastoma. For clinical application, radioiodinated MIBG is prepared through isotopic exchange method, which includes replacement of radioactive iodine in a...

A simple method for the determination of serum cholesterol

Improved Visualization of Bacterial Specimens for Ultrathin Sectioning.

Hashimoto and Naylor (J. Bacteriol., 75, 640, 1958) have shown that enclosing bacterial cells in agar prior to embedding the specimens in butyl methaerylate greatly decreases distortion and explosion of the cells. In using an agar-enclosing technique to study the relationship of cells in colonies of Bacillus anthracis, it was necessary to expose the colonies to osmium tetroxide after enclosing ...